Detects human CCR5 in Western blots. For additional information regarding epitope specificity for this antibody and other R&D Systems anti‑human CCR5 antibodies, see Reference 1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human/Mouse/Rat CCR5 APC-conjugated Antibody

Detection of CCR5 in Human PBMCs by Flow Cytometry.

Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e Alexa Fluor® 405-conjugated Monoclonal Antibody (Catalog # FAB100V) and either (A) Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) or (B) Mouse IgG₁Allophycocyanin Isotype Control (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR5 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with anti-CD3, anti-CD28, 5 ng/mL Recombinant Mouse IL-12 (Catalog # 419-ML) and 10 µg/mL Goat Anti-Mouse IL-4 Poly-clonal Antibody (Catalog # AB-404-NA) to induce Th1 development for 3 days were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of CCR5 in Rat Splenocytes by Flow Cytometry.

Rat splenocytes were stained with Mouse Anti-Human/Mouse/Rat CCR5 APC-conjugated Monoclonal Antibody (Catalog # FAB1802A) and Anti-Rat CD3 PE-conjugated Antibody. Quadrant markers were set based on control antibody staining (Catalog # IC002A). View our protocol for Staining Membrane-associated Proteins.

Applications for Human/Mouse/Rat CCR5 APC-conjugated Antibody

Application

Recommended Usage

Flow Cytometry

10 µL/10⁶ cells
Sample: Human peripheral blood mononuclear cells (PBMCs), mouse splenocytes treated with anti-CD3, anti-CD28, Recombinant Mouse IL‑12 (Catalog # 419-ML) and Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA) to induce Th1 development, and rat splenocytes

Reviewed Applications

Read 2 reviews rated 5 using FAB1802A in the following applications:

Flow Cytometry (2 Reviews)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

References

Lee, B. et al. (1999) J. Biol. Chem. 274:9617.

Alternate Names

CCR5, CD195

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Gene Symbol

CCR5

Additional CCR5 Products

Product Documents for Human/Mouse/Rat CCR5 APC-conjugated Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human/Mouse/Rat CCR5 APC-conjugated Antibody

For research use only

Citations
Reviews

Spectra Viewer
Flow Cytometry

Human/Mouse/Rat CCR5 APC-conjugated Antibody

R&D Systems, part of Bio-Techne | Catalog # FAB1802A

Key Product Details

Validated by

Species Reactivity

Validated:

Cited:

Applications