Human/Mouse/Rat CSRP1 Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5739

R&D Systems, part of Bio-Techne
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AF5739
AF5739-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

View all CSRP1 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CSRP1
Met1-Glu193
Accession # P21291

Specificity

Detects human, mouse, and rat CSRP1 in Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat CSRP1 Antibody

Detection of Human, Mouse, and Rat CSRP1 antibody by Western Blot.

Detection of Human, Mouse, and Rat CSRP1 by Western Blot.

Western blot shows lysates of ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.5 µg/mL Goat Anti-Human/Mouse/Rat CSRP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5739) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human CSRP1, CSRP2, CSRP3 (5 ng/lane) were included. A specific band for CSRP1 was detected at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat CSRP1 Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: ME-180 human cervical epithelial carcinoma cell line, HT-2 mouse T cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CSRP1

CSRP1 (Cysteine-rich protein 1; also CRP1 and Cysteine and Glycine‑rich protein 1) is a 23 kDa cytoplasmic member of the CRP family of LIM domain‑containing proteins. It is expressed in both visceral and vascular smooth muscle, and interacts with zyxin and alpha‑actinin, two proteins involved in actin assembly and crosslinking, during muscle cell differentiation. Human CSRP1 is 193 amino acids (aa) in length and contains two 50 aa LIM (for lin11/isl1/mec3) domains that contain two zinc‑finger motifs (aa 10‑61 and 119‑171), an NLS between aa 64‑69, and a Gly-rich C-terminus (aa 176‑187). There are two likely phosphorylation sites at Tyr127 and Ser192. Full‑length human CSRP1 shares 99% aa identity with mouse CSRP1.

Long Name

Cysteine-rich Protein 1

Alternate Names

CRP1, CYRP

Entrez Gene IDs

1465 (Human); 13007 (Mouse); 29276 (Rat)

Gene Symbol

CSRP1

UniProt

P21291

Additional CSRP1 Products

Product Documents for Human/Mouse/Rat CSRP1 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat CSRP1 Antibody

For research use only

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