Human/Mouse/Rat Cyclophilin A Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3589
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclophilin A
Met1-Glu165
Accession # P62937
Met1-Glu165
Accession # P62937
Specificity
Detects human, mouse, and rat Cyclophilin A in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Cyclophilin A Antibody
Detection of Human, Mouse, and Rat Cyclophilin A by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2-11 rat lymphoma cell line. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Cyclophilin A at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Cyclophilin A by Simple WesternTM.
Simple Western shows lysates of Exosome Standards (HT-29) (NBP3-11685), Exosome Standards (PC-3) (NBP2-49856) and MOLT-4 human acute lymphoblastic leukemia cell line, loaded at 0.5 mg/ml. A specific band was detected for Cyclophilin A at approximately 23 kDa (as indicated) using 50 µg/mL of Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.
Cyclophilin A in PANC-1 Human Cell Line.
Cyclophilin A was detected in immersion fixed PANC-1 human pancreatic carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3589) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Cyclophilin A Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed PANC-1 human pancreatic carcinoma cell line
Sample: Immersion fixed PANC-1 human pancreatic carcinoma cell line
Simple Western
5-50 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (PC-3) (Catalog # NBP2-49856), MOLT-4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685), Exosome Standards (PC-3) (Catalog # NBP2-49856), MOLT-4 human acute lymphoblastic leukemia cell line, HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Western Blot
0.1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, and Nb2‑11 rat lymphoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclophilin A
Cyclophilin A, also called Peptidyl-prolyl Isomerase A, PPIA, CYPA, and CYPH, was originally characterized for its ability to catalyze the transition between cis- and trans- proline residues critical for proper folding of proteins (1). Cyclophilin is also incorporated into many viruses, including HIV-1, where it has been speculated to be involved in functions such as viral assembly and infectivity (2). The immunosuppressive activity of cyclosporins has been correlated with their ability to form complexes with cyclophilins that inhibit calcineurin phosphatase activity (3) and prevent incorporation of cyclophilin into viral particles (4). The cyclosporin/cyclophilin complex selectively binds and inactivates calcineurin (3, 5), making it a useful inhibitor for studying calcineurin activity.
References
- Hamilton, G.S. and J.P. Steiner (1998) J. Med. Chem. 41:5119.
- Cantin, R. et al. (2005) J. Virology 79:6577.
- Liu, J. et al. (1992) Biochemistry 31:3896.
- Wiegers K. and H.G. Krausslich (2002) Virology 294:289.
- Liu, J. et al. (1991) Cell 66:807.
Alternate Names
CYPA, CYPH, PPIA, Rotamase
Gene Symbol
PPIA
UniProt
Additional Cyclophilin A Products
Product Documents for Human/Mouse/Rat Cyclophilin A Antibody
Product Specific Notices for Human/Mouse/Rat Cyclophilin A Antibody
For research use only
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