Human/Mouse/Rat Cyclophilin B Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5410
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Knockout Validated, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Cyclophilin B
Asp34-Glu216
Accession # P23284
Asp34-Glu216
Accession # P23284
Specificity
Detects human, mouse, and rat Cyclophilin B in Western blots. In Western blots and direct ELISAs, less than 1% cross-reactivity with recombinant human Cyclophilin A is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Cyclophilin B Antibody
Detection of Human/Mouse/Rat Cyclophilin B by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). For additional reference, recombinant human Cyclophilin A and Cyclophilin B (5 ng/lane) were included. A specific band for Cyclophilin B was detected at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Cyclophilin B in HeLa Human Cell Line.
Cyclophilin B was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Cyclophilin B in HeLa Human Cell Line.
Cyclophilin B was detected in immersion fixed wildtype (left panel) but is not detected in Cyclophilin B knockout (right panel) HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human/Mouse/Rat Cyclophilin B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5410) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Cyclophilin B Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Knockout Validated
Cyclophilin B is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Cyclophilin B knockout HeLa cell line.
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line
Sample: HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, BaF3 mouse pro-B cell line, and Y3-Ag rat myeloid cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Cyclophilin B
Cyclophilin B (SCYLP, CyPB and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization, and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204) and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical.
Alternate Names
CYP-S1, CYPB, PPIB, S-cyclophilin, SCYLP
Gene Symbol
PPIB
UniProt
Additional Cyclophilin B Products
Product Documents for Human/Mouse/Rat Cyclophilin B Antibody
Product Specific Notices for Human/Mouse/Rat Cyclophilin B Antibody
For research use only
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