Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3564
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Dopa Decarboxylase/DDC
Met1-Glu480
Accession # P20711
Met1-Glu480
Accession # P20711
Specificity
Detects human Dopa Decarboxylase/DDC in direct ELISAs and human, mouse and rat Dopa Decarboxylase/DDC in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody
Detection of Human, Mouse, and Rat Dopa Decarboxylase/DDC by Western Blot.
Western blot shows lysates of mouse kidney tissue, PC-12 rat adrenal pheochromocytoma cell line, and human kidney tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Dopa Decarboxylase/DDC in IMR‑32 Human Cell Line.
Dopa Decarboxylase/DDC was detected in immersion fixed IMR-32 human neuroblastoma cell line using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Dopa Decarboxylase/DDC in Human Brain.
Dopa Decarboxylase/DDC was detected in immersion fixed paraffin-embedded sections of human brain (substantia nigra) using Goat Anti-Human/Mouse/Rat Dopa Decarboxylase/DDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3564) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed IMR-32 human neuroblastoma cell line
Sample: Immersion fixed IMR-32 human neuroblastoma cell line
Immunohistochemistry
1-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and perfusion fixed frozen sections of mouse brain
Sample: Immersion fixed paraffin-embedded sections of human brain (substantia nigra) and perfusion fixed frozen sections of mouse brain
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Dopa Decarboxylase/DDC (Catalog # 3564-DC), see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human Dopa Decarboxylase/DDC (Catalog # 3564-DC), see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Mouse kidney tissue, PC‑12 rat adrenal pheochromocytoma cell line, and human kidney tissue
Sample: Mouse kidney tissue, PC‑12 rat adrenal pheochromocytoma cell line, and human kidney tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Dopa Decarboxylase/DDC
Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).
References
- Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
- Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.
Alternate Names
AADC, DDC
Entrez Gene IDs
1644 (Human)
Gene Symbol
DDC
UniProt
Additional Dopa Decarboxylase/DDC Products
Product Documents for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody
Product Specific Notices for Human/Mouse/Rat Dopa Decarboxylase/DDC Antibody
For research use only
Loading...
Loading...
Loading...
Loading...