Human/Mouse/Rat eEF-2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3806
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human eEF-2
Val300-Ser500
Accession # Q6PK56
Val300-Ser500
Accession # Q6PK56
Specificity
Detects human, mouse, and rat eEF-2 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat eEF-2 Antibody
Detection of Human/Mouse/Rat eEF-2 by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell line, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat eEF-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3806) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for eEF-2 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat eEF-2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, U2OS human osteosarcoma cell line, and Balb/3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: eEF-2
The eukaryotic translation elongation factor-2 (eEF-2) is a GTP-binding protein that is required for the translocation step from the A site to the P site of the ribosome. Phosphorylation of eEF-2 by the eEF-2 kinase causes complete inhibition of its activity.
Long Name
Eukaryotic Translation Elongation Factor 2
Alternate Names
eEF2
Gene Symbol
EEF2
UniProt
Additional eEF-2 Products
Product Documents for Human/Mouse/Rat eEF-2 Antibody
Product Specific Notices for Human/Mouse/Rat eEF-2 Antibody
For research use only
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