Human/Mouse/Rat EMP/MAEA Antibody

Detection of Human, Mouse, and Rat EMP/MAEA by Western Blot.

Western blot shows lysates of C2C12 mouse myoblast cell line, Jurkat human acute T cell leukemia cell line, K562 human chronic myelogenous leukemia cell line, and NR8383 rat alveolar macrophage cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for EMP/MAEA at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

EMP/MAEA in Jurkat Human Cell Line.

EMP/MAEA was detected in immersion fixed Jurkat human acute T cell leukemia cell line using Sheep Anti-Human EMP/MAEA Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7288) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Detection of Mouse EMP/MAEA by Western Blot

V5-HA-tagged RanBP9 maintains its ability to interact with known members of the CTLH complex and Nucleolin. RanBP9 WT and TT immortalized Mouse Embryonic Fibroblasts (MEFs) were cultured in standard conditions and protein lysates were obtained. Resin conjugated with alphaHA antibodies was used to immunoprecipitate RanBP9-TT protein. IPed fractions and 5% of input were run on gels to generate 5 different membranes that were probed with the indicated antibodies by WB. Vinculin is used as loading control. Shown results are representative of two independent experiments (biological replicates). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32346083), licensed under a CC-BY license. Not internally tested by R&D Systems.