Human/Mouse/Rat FABP1/L-FABP Antibody

Detection of Human, Mouse, and Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysates of H4-II-E-C3 rat hepatoma cell line, mouse liver tissue, human liver tissue, and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/ Mouse/Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Rat FABP1/L‑FABP by Western Blot.

Western blot shows lysate of rat liver tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/ Rat FABP1/L-FABP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for FABP1/ L-FABP at approximately 13 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

FABP1 in Rat Liver.

FABP1 was detected in perfusion fixed frozen sections of rat liver using Goat Anti-Human/Mouse/Rat FABP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1565) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.