Human/Mouse/Rat FABP3/H-FABP Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1678
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 242305
Product Specifications
Immunogen
E. coli-derived recombinant human FABP3/H‑FABP
Met1-Ala133
Accession # P05413
Met1-Ala133
Accession # P05413
Specificity
Detects human, mouse, and rat FABP3/H‑FABP in Western blots. In Western blots, approximately 15% cross-reactivity with recombinant rat (rr) FABP2 is observed and no cross-reactivity with rrFABP1, recombinant mouse (rm) FABP4, or rmFABP5 is observed.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat FABP3/H-FABP Antibody
Detection of Human, Mouse, and Rat FABP3/H‑FABP by Western Blot.
Western blot shows lysates of human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for FABP3/H-FABP at approximately 14 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
FABP3/H‑FABP in Human Heart.
FABP3/H-FABP was detected in immersion fixed paraffin-embedded sections of human heart using Rat Anti-Human/Mouse/Rat FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counter-stained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Human and Mouse FABP3/H‑FABP by Simple WesternTM.
Simple Western lane view shows lysates of human heart tissue and mouse heart tissue, loaded at 0.2 mg/mL. A specific band was detected for FABP3/H-FABP at approximately 19 kDa (as indicated) using 5 µg/mL of Rat Anti-Human FABP3/H-FABP Monoclonal Antibody (Catalog # MAB1678) followed by 1:50 dilution of HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat FABP3/H-FABP Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human heart
Sample: Immersion fixed paraffin-embedded sections of human heart
Simple Western
5 µg/mL
Sample: Human heart tissue and mouse heart tissue
Sample: Human heart tissue and mouse heart tissue
Western Blot
0.5 µg/mL
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue
Sample: Human heart tissue, human skeletal muscle tissue, mouse heart tissue, and rat heart tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB1678 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FABP3/H-FABP
Long Name
Fatty Acid-Binding Protein 3
Alternate Names
H-FABP, HFABP, MDGI
Entrez Gene IDs
2170 (Human)
Gene Symbol
FABP3
UniProt
Additional FABP3/H-FABP Products
Product Documents for Human/Mouse/Rat FABP3/H-FABP Antibody
Product Specific Notices for Human/Mouse/Rat FABP3/H-FABP Antibody
For research use only
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