Human/Mouse/Rat Fibromodulin/FMOD Antibody

R&D Systems, part of Bio-Techne | Catalog # AF5945

R&D Systems, part of Bio-Techne
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AF5945
AF5945-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Fish - Danio rerio (Zebrafish)

Applications

Validated:

Western Blot

Cited:

Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG

View all Fibromodulin/FMOD Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Fibromodulin/FMOD
Asp75-Ile376
Accession # Q06828

Specificity

Detects human, mouse, and rat Fibromodulin/FMOD in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Fibromodulin/FMOD Antibody

Detection of Human/Mouse/Rat Fibromodulin antibody by Western Blot.

Detection of Human/Mouse/Rat Fibromodulin by Western Blot.

Western blot shows lysates of human cartilage, human ovarian cancer, and rat uterine tissue. PVDF membrane was probed with 0.3 µg/mL of Sheep Anti-Human/Mouse/Rat Fibromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5945) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Fibromodulin at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human/Mouse/Rat Fibromodulin/FMOD Antibody

Application
Recommended Usage

Western Blot

0.3 µg/mL
Sample: Human cartilage, human ovarian cancer, and rat uterine tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fibromodulin/FMOD

Fibromodulin (FMOD; also FM and collagen-binding 59 kDa protein) is a secreted, 60‑70 kDa class II member of the SLRP family of matrix proteins. It is expressed by a variety of cell types, including keratinocytes, fibroblasts and chrondrocytes, and is known to bind to type I and XII collagen, plus TGF-beta. Fibromodulin is posited to displace lumican binding to collagen, generating 3D collagen fibrils from simple lumican-induced collagen dimers. Mature human Fibromodulin is 358 amino acids (aa) in length (aa 19‑376). It contains 12 LRRs (Leu-rich repeats) (aa 98‑376), with the seventh and eleventh LRR principally responsible for collagen type I binding. Fibromodulin contains varying amounts of keratan sulfate, and undergoes significant tyrosine sulfation. There is one splice form that shows a deletion of aa 88‑101. Over aa 76‑376, human Fibromodulin shares 96% aa identity with mouse and rat Fibromodulin.

Alternate Names

FM, FMOD, SLRR2E

Entrez Gene IDs

2331 (Human); 14264 (Mouse); 64507 (Rat)

Gene Symbol

FMOD

UniProt

Q06828

Additional Fibromodulin/FMOD Products

Product Documents for Human/Mouse/Rat Fibromodulin/FMOD Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Fibromodulin/FMOD Antibody

For research use only

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