Human/Mouse/Rat FKBP13 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4356
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human FKBP13
Ala22-Leu142
Accession # P26885
Ala22-Leu142
Accession # P26885
Specificity
Detects endogenous human, mouse, and rat FKBP13 in Western blots. In Western blots, this antibody does not cross‑react with recombinant human FKBP12, FKBP25, FKBP38, FKBP51 or FKBP52.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat FKBP13 Antibody
Detection of Human/Mouse/Rat FKBP13 by Western Blot.
Western blot shows lysates of Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat FKBP13 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4356) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FKBP13 at approximately 13 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat FKBP13 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line
Sample: Rat-2 rat embryonic fibroblast cell line and Neuro-2A mouse neuroblastoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FKBP13
FK506 binding protein, 13 kilodalton molecular weight (FKBP13), also called FKBP2, is a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans‑proline residues critical for proper folding of proteins. The immunosuppressants FK506 and rapamycin are potent inhibitors of FKBP13. This isomerase is localized in the endoplasmic reticulum and is also associated with cytoskeletal elements such as erythrocyte band 4.1. FKBP13 levels are increased by treatments that affect golgi protein processing such as tunicamycin and heat shock, suggesting that FKBP13 is important in the unfolded protein response observed with endoplasmic reticulum stress.
Long Name
13 kDa FK506 Binding Protein
Alternate Names
FKBP2
Gene Symbol
FKBP2
UniProt
Additional FKBP13 Products
Product Documents for Human/Mouse/Rat FKBP13 Antibody
Product Specific Notices for Human/Mouse/Rat FKBP13 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...