Human/Mouse/Rat GABA-A R alpha 4 N-Terminus Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS026

R&D Systems, part of Bio-Techne
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PPS026
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all GABA-A R alpha 4 Antibodies »

Product Specifications

Immunogen

Peptide from GABAA R alpha4 subunit, N-Terminus conjugated to keyhole limpet hemocyanin (KLH)

Specificity

Human, mouse, rat, and bovine ~64 kDa GABAA R alpha4 subunit, N-Terminus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat GABA-A R alpha 4 N-Terminus Antibody

Detection of GABAAR a4 N-Terminus antibody by Western Blot.

Detection of GABAAR alpha4 N-Terminus by Western Blot.

Western blot of rat brain (hippocampal) lysates using approximately 5 - 7 μg of tissue per lane. The blot was incubated overnight at 2 - 8° C with anti-GABAAR, alpha4 subunit, N-Terminus diluted 1:1000. The antibody labeled the ~64 kDa alpha4 subunit of the GABAAR.

Applications for Human/Mouse/Rat GABA-A R alpha 4 N-Terminus Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution
Sample: Rat brain (hippocampus) tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R alpha 4

GABAA ( gamma-aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABAA receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, delta, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABAA receptors have some combination of alpha, beta and gamma subunits. The rat alpha4 isoform is a 67 kDa, 517 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 14 - 221). The alpha4 subunit is unusual in that it does not seem to preferentially form channel complexes with the gamma subunit. In hippocampal neurons, alpha4 subunits apparently form GABA receptors that do not cluster, but exist in diffuse net-works in nonsynaptic membranes.

References

  1. Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
  2. Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
  3. Wisden, W. et al. (1991) FEBS Lett. 289:227.
  4. Bencsits, E. et al. (1999) J. Biol. Chem. 274:19613.
  5. Mangan, P.S. et al. (2005) Mol. Pharmacol. 67:775.

Long Name

gamma-Aminobutyric Acid Type A Receptor, alpha-4 Polypeptide

Alternate Names

GABAAR alpha 4, GABAARa4, GABRA4

Entrez Gene IDs

2557 (Human); 14397 (Mouse); 140675 (Rat)

Gene Symbol

GABRA4

Additional GABA-A R alpha 4 Products

Product Documents for Human/Mouse/Rat GABA-A R alpha 4 N-Terminus Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Human/Mouse/Rat GABA-A R alpha 4 N-Terminus Antibody

For research use only

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