Human/Mouse/Rat GAD1/GAD67 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2086
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse, Transgenic Mouse
Applications
Validated:
Immunohistochemistry, Simple Western, Western Blot
Cited:
Immunohistochemistry, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GAD1/GAD67
Ala2-Asn97
Accession # Q99259
Ala2-Asn97
Accession # Q99259
Specificity
Detects human GAD1/GAD67 in direct ELISAs and detects human, mouse, and rat GAD1/GAD67 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GAD1/GAD67 Antibody
Detection of Human, Mouse, and Rat GAD1/GAD67 by Western Blot.
Western blot shows lysates of human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody HAF017). A specific band was detected for GAD1/GAD67 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
GAD1/GAD67 in Human Spinal Cord.
GAD1/GAD67 was detected in immersion fixed paraffin-embedded sections of human spinal cord using 15 µg/mL Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Detection of Mouse GAD1/GAD67 by Simple WesternTM.
Simple Western lane view shows lysates of mouse brain tissue, loaded at 0.2 mg/mL. A specific band was detected for GAD1/GAD67 at approximately 65 kDa (as indicated) using 1 µg/mL of Goat Anti-Human/Mouse/Rat GAD1/GAD67 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2086) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat GAD1/GAD67 Antibody
Application
Recommended Usage
Immunohistochemistry
5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Sample: Immersion fixed paraffin-embedded sections of human spinal cord
Simple Western
1 µg/mL
Sample: Mouse brain tissue
Sample: Mouse brain tissue
Western Blot
1 µg/mL
Sample: Human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue
Sample: Human brain (hypothalamus) tissue, mouse brain (total) tissue, and rat brain (total) tissue
Reviewed Applications
Read 1 review rated 1 using AF2086 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GAD1/GAD67
Long Name
Glutamate Decarboxylase 1 (Brain, 67kDa)
Alternate Names
GAD67
Entrez Gene IDs
2571 (Human)
Gene Symbol
GAD1
UniProt
Additional GAD1/GAD67 Products
Product Documents for Human/Mouse/Rat GAD1/GAD67 Antibody
Product Specific Notices for Human/Mouse/Rat GAD1/GAD67 Antibody
For research use only
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