Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

R&D Systems, part of Bio-Techne | Catalog # PPS055

R&D Systems, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

View all GRIN2B/NMDAR2B Antibodies »

Product Specifications

Immunogen

NMDA R, NR2B Subunit

Specificity

Human, mouse, and rat ~180 kDa NMDA NR2B

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Scientific Data Images for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Detection of GRIN2B/NMDAR2B antibody by Western Blot.

Detection of GRIN2B/NMDAR2B by Western Blot.

Western blot of rat hippocampal lysate showing specific immunolabeling of the approximately 180 kDa NR2B subunit of the NMDA Receptor.

Applications for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Application
Recommended Usage

Western Blot

1:1000 dilution

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

50 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 mg/mL BSA and 50% glycerol.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage,  -20 °C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GRIN2B/NMDAR2B

NMDA (N-Methyl D-Aspartate) receptors are members of the glutamate receptor family of ligand-gated ion channels. The functional NMDA receptor (NMDAR) is a 650-850 kDa heteromultimer of at least two NR2 (NMDAR2) subunits and two NR1 subunits. NR2 subunits determine overall NMDAR characteristics such as conductance and Mg++ sensitivity. In addition, NR2 subunits mediate NMDAR clustering and synaptic localization through cytoplasmic interaction with PSD-95/SAP90 family members. Upon glutamate binding to NR2, and glycine binding to NR1, the NMDA channel is opened, allowing calcium and sodium influx into the cell. There are four genes that code for NR2 subunits (NR2A-D). The two (or three) NR2 subunits making up the NMDAR may be homodimers or heterodimers. The 2B form generates a high conductance NMDAR. Human NR2B (also NR3 and GRIN2B) is a 180 kDa, 1458 amino acid (aa), three transmembrane (TM) glycoprotein that contains a 531 aa N-terminal extracellular domain (ECD) and a 646 aa cytoplasmic region. The loop connecting TM segments 2 and 3, plus the N-terminal ECD constitute the glutamate binding site. Human NR2B is more than 98% aa identical to NR2B in mouse, rat, and dog.

References

  1. Stephenson, F.A. (2001) Curr. Drug Targets 2:233.
  2. Cull-candy, S.G. and D.N. Leszkiewicz (2004) Sci. STKE re16 (2004).
  3. Prybylowski, K. and R.J. Wenthold (2004) J. Biol. Chem. 279:9673.
  4. Ishii, T. et al. (1993) J. Biol. Chem. 268:2836.
  5. Hess, S.D. et al. (1996) J. Pharmacol. Exp. Ther. 278:808.

Long Name

Glutamate Receptor, Ionotropic, N-Methyl-D-Aspartate, Subunit 2B

Alternate Names

NMDA R, NR2B Subunit, NMDAR2B, NR2B

Entrez Gene IDs

2904 (Human)

Gene Symbol

GRIN2B

Additional GRIN2B/NMDAR2B Products

Product Documents for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat GRIN2B/NMDAR2B Antibody

For research use only

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