Human/Mouse/Rat GRK5 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4539
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Knockout Validated, Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947
Pro463-Ser590
Accession # P34947
Specificity
Detects endogenous human, mouse, and rat GRK5 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat GRK5 Antibody
Detection of Human/Mouse/Rat GRK5 by Western Blot.
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for GRK5 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western Blot Shows Human GRK5 Specificity by Using Knockout Cell Line.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat GRK5 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4539) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for GRK5 at approximately 65 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat GRK5 Antibody
Application
Recommended Usage
Knockout Validated
GRK5
is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in
GRK5 knockout HeLa cell line.
Western Blot
0.5 µg/mL
Sample: Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line
Sample: Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line
Reviewed Applications
Read 1 review rated 4 using AF4539 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GRK5
G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.
Long Name
G protein-coupled Receptor Kinase 5
Alternate Names
GPRK5
Gene Symbol
GRK5
UniProt
Additional GRK5 Products
Product Documents for Human/Mouse/Rat GRK5 Antibody
Product Specific Notices for Human/Mouse/Rat GRK5 Antibody
For research use only
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