Human/Mouse/Rat Histone H2AY Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB8318
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 866416
Product Specifications
Immunogen
E. coli-derived recombinant human Histone H2AY
Gly230-Thr348
Accession # O75367
Gly230-Thr348
Accession # O75367
Specificity
Detects human Histone H2AY in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat Histone H2AY Antibody
Detection of Human, Mouse, and Rat Histone H2AY by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line, Hepa 1-6 mouse hepatoma cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Histone H2AY at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Histone H2AY in HeLa Human Cell Line.
Histone H2AY was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat Histone H2AY Monoclonal Antibody (Catalog # MAB8318) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat Histone H2AY Antibody
Application
Recommended Usage
Immunocytochemistry
8-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line
Western Blot
2 µg/mL
Sample: MCF‑7 human breast cancer cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Sample: MCF‑7 human breast cancer cell line, Hepa 1‑6 mouse hepatoma cell line, and Rat‑2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Histone H2AY
Long Name
H2A Histone Family, Member Y
Alternate Names
H2AF12M, H2AFJ, H2AFY, Histone MacroH2A1, Medulloblastoma Antigen MU-MB-50.205
Gene Symbol
MACROH2A1
UniProt
Additional Histone H2AY Products
Product Documents for Human/Mouse/Rat Histone H2AY Antibody
Product Specific Notices for Human/Mouse/Rat Histone H2AY Antibody
For research use only
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