Human/Mouse/Rat HO-2/HMOX2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3170
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human HO-2/HMOX2
Met1-Met316
Accession # P30519
Met1-Met316
Accession # P30519
Specificity
The antibody is known to react with endogenous human, mouse, and rat HO-2 in in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat HO-2/HMOX2 Antibody
Detection of Human/Mouse/Rat HO-2/HMOX2 by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for HO-2/HMOX2 at approximately 38 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human, Mouse, and Rat HO‑2/HMOX2 by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, PC-12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Mouse HO‑2/HMOX2 by Simple WesternTM.
Simple Western lane view shows lysates of DA3 mouse myeloma cell line and C2C12 mouse myoblast cell line, loaded at 0.2 mg/mL. A specific band was detected for HO-2/HMOX2 at approximately 43 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat HO-2/HMOX2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3170) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat HO-2/HMOX2 Antibody
Application
Recommended Usage
Simple Western
5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line
Sample: HeLa human cervical epithelial carcinoma cell line, PC‑12 rat adrenal pheochromocytoma cell line, DA3 mouse myeloma cell line, and C2C12 mouse myoblast cell line
Western Blot
0.5 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line
Sample: Jurkat human acute T cell leukemia cell line, HeLa human cervical epithelial carcinoma cell line, C2C12 mouse myoblast cell line, DA3 mouse myeloma cell line, and PC-12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: HO-2/HMOX2
Heme Oxygenase 2 (HO-2), also known as HMOX2, is a 36 kDa microsomal enzyme required for the metabolism of heme to biliverdin. Heme oxygenase occurs as 2 isozymes, the constitutively expressed heme oxygenase-2 (HO-2/HMOX2) and the inducible heme oxygenase-1 (HO-1/HMOX1). HO-1 expression is induced by heme and other non-heme compounds. Human HO-2 shares 42% amino acid sequence identity with human HO-1 and 89% amino acid sequence identity with mouse and rat HO-2.
Long Name
Heme Oxygenase 2
Alternate Names
HMOX2, HO2
Gene Symbol
HMOX2
UniProt
Additional HO-2/HMOX2 Products
Product Documents for Human/Mouse/Rat HO-2/HMOX2 Antibody
Product Specific Notices for Human/Mouse/Rat HO-2/HMOX2 Antibody
For research use only
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