Human/Mouse/Rat ILK Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4266
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 443208
Product Specifications
Immunogen
E. coli-derived recombinant human ILK
Met1-Lys452
Accession # Q13418
Met1-Lys452
Accession # Q13418
Specificity
Detects human, mouse, and rat ILK in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat ILK Antibody
Detection of Human/Mouse/Rat ILK by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat ILK Monoclonal Antibody (Catalog # MAB4266) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human ILK (1 ng) was included. A specific band for ILK was detected at approximately 59 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat ILK Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line
Sample: HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, C2C12 mouse myoblast cell line, and PT18 mouse mast/basophil cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 5 reviews rated 4.4 using MAB4266 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ILK
Integrin-linked kinase (ILK) is an ankyrin repeat-containing serine/threonine protein kinase that interacts with the cytoplasmic domain of beta1 and beta3 integrins. ILK functions with multiple binding partners to transmit signals from the extracellular matrix to actin filaments, regulating such processes as cellular attachment, cell cycle progression, and cytoskeletal organization. ILK kinase activity is induced by PI3 kinase and negatively regulated by the tumor suppressor PTEN.
Long Name
Integrin-linked Kinase
Alternate Names
ESTM24, p59
Gene Symbol
ILK
UniProt
Additional ILK Products
Product Documents for Human/Mouse/Rat ILK Antibody
Product Specific Notices for Human/Mouse/Rat ILK Antibody
For research use only
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