Human/Mouse/Rat ILKAP/PP2C delta Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4554
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 499526
Product Specifications
Immunogen
E. coli-derived recombinant human ILKAP
Met1-His392
Accession # Q9H0C8
Met1-His392
Accession # Q9H0C8
Specificity
Detects endogenous human, mouse and rat ILKAP in Western blots. In Western blots, this antibody does not cross-react with recombinant human PP2C alpha, PP2C beta or PPC2 gamma.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Detection of Human ILKAP/PP2C delta by Western Blot.
Western blot shows lysates of PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat ILKAP/PP2Cd Monoclonal Antibody (Catalog # MAB4554) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ILKAP/PP2Cd at approximately 47 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line
Sample: PC-3 human prostate cancer cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ILKAP/PP2C delta
Integrin Linked Kinase 1 Associated Phosphatase (ILKAP), also called PP2C delta, is a serine/threonine phosphatase that is stimulated by manganese but inhibited by magnesium ions and is insensitive to the phosphatase inhibitor okadaic acid. It binds to ILK1 and dephosphorylates it, decreasing the stimulation of the GSK-3 beta pathway by cell adhesion without affecting Akt signaling. Transfection studies have shown that ILKAP activity inhibits cell cycle progression from the G1 to S phase. ILKAP is expressed in many tissues but is highest in liver, and skeletal and cardiac muscle. Its gene is found in a region of chromosome 2 that is frequently deleted in oral cancers.
Long Name
Integrin-Linked Kinase-Associated Serine/Threonine Phosphatase 2C
Alternate Names
PP2C delta
Gene Symbol
ILKAP
UniProt
Additional ILKAP/PP2C delta Products
Product Documents for Human/Mouse/Rat ILKAP/PP2C delta Antibody
Product Specific Notices for Human/Mouse/Rat ILKAP/PP2C delta Antibody
For research use only
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