Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8554
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf21 derived recombinaint human IMP Dehydrogenase 1/IMPDH1
Met1-Tyr563 (predicted)
Accession # P20839
Met1-Tyr563 (predicted)
Accession # P20839
Specificity
Detects human, rat, and mouse IMP Dehydrogenase 1/IMPDH1 in direct ELISAs and Western blots. In direct ELISAs, less than5% cross-reactivity with recombinant mouse IMPDH2 is observed.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Detection of Human and Mouse IMP Dehydrogenase 1/IMPDH1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). Specific bands were detected for IMP Dehydrogenase 1/IMPDH1 at approximately 55-57 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
IMP Dehydrogenase 1/IMPDH1 in HeLa Human Cervical Epithelial Carcinoma Cell Line.
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at a 1:200 dilution for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI(blue). Specific staining was localized to cytoplasm and nucleus. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
IMP Dehydrogenase 1/IMPDH1 in C2C12 Mouse Cell Line.
IMP Dehydrogenase 1/IMPDH1 was detected in immersion fixed C2C12 mouse myoblast cell line using Rabbit Anti-Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8554) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.Applications for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Application
Recommended Usage
Immunocytochemistry
1:200 dilution
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line and C2C12 mouse myoblast cell line
Simple Western
2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line
Sample: HeLa human cervical epithelial carcinoma cell line
Western Blot
1:1000 dilution
Sample: HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line
Sample: HeLa human cervical epithelial carcinoma cell line and BaF3 mouse pro-B cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C, as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 to -70 °C under sterile conditions after opening.
Background: IMP Dehydrogenase 1/IMPDH1
Long Name
Inosine 5'-Monophosphate Dehydrogenase 1
Alternate Names
IMPD1, IMPDH1, LCA11, RP10
Gene Symbol
IMPDH1
UniProt
Additional IMP Dehydrogenase 1/IMPDH1 Products
Product Documents for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
Product Specific Notices for Human/Mouse/Rat IMP Dehydrogenase 1/IMPDH1 Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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