Human/Mouse/Rat iNOS Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB9502

R&D Systems, part of Bio-Techne
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MAB9502
MAB9502-SP
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, N/A, Rabbit, Xenograft

Applications

Validated:

Immunohistochemistry, Simple Western, Western Blot

Cited:

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 2D2-B2

View all iNOS Antibodies »

Product Specifications

Immunogen

Recombinant human iNOS
Pro781-His798
Accession # P35228

Specificity

Detects human iNOS. By using synthetic peptides, the epitope recognized by this antibody has been mapped to aa 781-798 of human iNOS. The corresponding sequence of mouse iNOS is identical.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human/Mouse/Rat iNOS Antibody

Detection of Human iNOS antibody by Western Blot.

Detection of Human iNOS by Western Blot.

Western blot shows lysates of DLD clone 2C2 human colon adenocarcinoma cell line untreated (-) or treated (+) with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # HAF007). A specific band was detected for iNOS at approximately 130 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Detection of Mouse and Rat iNOS antibody by Western Blot.

Detection of Mouse and Rat iNOS by Western Blot.

Western blot shows lysates of RAW 264.7 mouse monocyte/macrophage cell line and NR8383 rat alveolar macrophage cell line untreated (-) or treated (+) with 10 µg/mL LPS for 4 hours. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for iNOS at approximately 125 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
iNOS antibody in Human Brain by Immunohistochemistry (IHC-P).

iNOS in Human Brain.

iNOS was detected in immersion fixed paraffin-embedded sections of human brain (medulla) using Mouse Anti-Human/Mouse/Rat iNOS Monoclonal Antibody (Catalog # MAB9502) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
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Applications for Human/Mouse/Rat iNOS Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human brain (medulla).

Simple Western

10 µg/mL
Sample: RAW 264.7 mouse monocyte/macrophage cell line treated with LPS

Western Blot

1 µg/mL
Sample: DLD clone 2C2 human colon adenocarcinoma cell line treated with Recombinant Human IL-1 beta, Recombinant Human TNF-alpha, and Recombinant Human IFN-gamma (Catalog # 201-LB, 210-TA, 285-IF), RAW 264.7 mouse monocyte/macrophage cell line treated with LPS, and NR8383 rat alveolar macrophage cell line treated with LPS
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 8 reviews rated 4 using MAB9502 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: iNOS

Inducible nitric oxide synthase (iNOS) is a widely expressed, approximately 130 kDa enzyme that catalyzes the conversion of L-arginine to nitric oxide (NO) plus citrulline. NO is a bioactive mediator that plays an important role in hemodynamics by inducing vascular smooth muscle relaxation. iNOS is constitutively expressed in the kidney. In other tissues, it is inducibly expressed during inflammation, oxidative stress, and hyperglycemia. iNOS activity is elevated in a variety of disease states including atherosclerosis, heart failure, sepsis, solid tumors, and type 2 diabetes. Increased production of NO mediates acute kidney injury, TNF-alpha induced muscle wasting, and the increased radiosensitivity of hypoxic tumor cells. Within amino acids 781-798, human iNOS shares 89% and 72% aa sequence identity with mouse and rat iNOS, respectively.

Long Name

Inducible Nitic Oxide Synthase

Alternate Names

NOS2, NOS2A

Entrez Gene IDs

4843 (Human); 18126 (Mouse)

Gene Symbol

NOS2

UniProt

P35228

Additional iNOS Products

Product Documents for Human/Mouse/Rat iNOS Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat iNOS Antibody

For research use only

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