Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB8050

Recombinant Monoclonal Antibody.
R&D Systems, part of Bio-Techne
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MAB8050-100
MAB8050-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2493A

View all Kynurenine 3-Monooxygenase/KMO Antibodies »

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Kynurenine 3-Monooxygenase/KMO
Asp2-Leu441
Accession # O15229

Specificity

Detects human Kynurenine 3-Monooxygenase/KMO in direct ELISAs. Detects human, mouse, and rat Kynurenine 3-Monooxygenase/KMO in Western blot.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO antibody by Western Blot.

Detection of Human, Mouse, and Rat Kynurenine 3-Monooxygenase/KMO by Western Blot.

Western blot shows lysates of mouse liver tissue, rat spleen tissue, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Kynurenine 3-Monooxygenase/KMO at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Kynurenine 3-Monooxygenase/KMO antibody in Human Placenta by Immunohistochemistry (IHC-P).

Kynurenine 3-Monooxygenase/KMO in Human Placenta.

Kynurenine 3-Monooxygenase/KMO was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Monoclonal Antibody (Catalog # MAB8050) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in syncytiotrophoblast cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Applications for Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

Application
Recommended Usage

Immunohistochemistry

3-25 µg/mL
Sample: Iimmersion fixed paraffin-embedded sections of human placenta

Western Blot

1 µg/mL
Sample: Mouse liver tissue, Rat spleen tissue, and MDA‑MB‑453 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kynurenine 3-Monooxygenase/KMO

Kynurenine 3-Monooxygenase (KMO), also known as Kynurenine 3-Hydroxylase, is a part of the kynurenine pathway of tryptophan degradation (1). KMO catalyzes the NADPH- and flavin adenine dinucleotide (FAD)-dependent 3-hydroxylation of kynurenine to 3-hydroxykynurenine (3-HK). 3-HK is neurotoxic via the generation of hydrogen peroxide (2) and through the excitotoxic effects of its downstream metabolite quinolinic acid (3). The levels of 3-HK and quinolinic acid are increased in the brain with Alzheimer's disease and Huntington's disease (1). Inhibition of KMO was shown to reverse cognitive and motor deficits in mouse models of those diseases via an increase in neuroprotective kynurenic acid (4). KMO is found in the mitochondrial outer membrane of microglial cells in the brain and dendritic cells and macrophages in the periphery (1). 

References

  1. Schwarcz, R. et al. (2012) Nat. Rev. Neurosci. 13:465.
  2. Okuda, S. et al. (1996) Proc. Natl. Acad. Sci. 93:12553.
  3. Stone, T.W. and M.N. Perkins. (1981) Eur. J. Pharmacol. 72:411.
  4. Zwilling, D. et al. (2011) Cell 145:863.

Alternate Names

KMO, Kynurenine 3Monooxygenase

Entrez Gene IDs

8564 (Human); 98256 (Mouse); 59113 (Rat)

Gene Symbol

KMO

UniProt

O15229

Additional Kynurenine 3-Monooxygenase/KMO Products

Product Documents for Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat Kynurenine 3-Monooxygenase/KMO Antibody

For research use only

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