Human/Mouse/Rat LAR Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3004
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human LAR
Ala27-Glu1251
Accession # NP_569707
Ala27-Glu1251
Accession # NP_569707
Specificity
Detects human, mouse, and rat LAR in Western blots. In Western blots, this antibody detects both full-length protein and the cleaved LAR extracellular (E) domain. The antibody does not cross react with PTPR sigma.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat LAR Antibody
Detection of Human/Mouse/Rat LAR by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat LAR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3004) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Bands were detected for LAR at approximately 207 kDa for full-length LAR and 139 kDa for cleaved LAR (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat LAR Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line
Sample: Raji human Burkitt's lymphoma cell line, Neuro-2A mouse neuroblastoma cell line, and Y3-Ag rat myeloid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF3004 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LAR
Leucocyte Antigen-Related (LAR) tyrosine phosphatase, also known as Protein Tyrosine Phoshatase, Receptor-type F (PTPRF), is an integral membrane protein with a non-glycosylated molecular weight of 207 kDa. The extracellular domain is cleaved near the cell membrane by a subtilisin-like endoprotease to a molecular weight of 139 kDa. Depending on cellular conditions, the extracellular domain may remain associated with the rest of the molecule or can be shed into the extracellular medium. This antibody was made against the extracellular domain of LAR and detects both full-length and cleaved LAR.
Long Name
Leukocyte Antigen-Related Tyrosine Phosphatase
Alternate Names
PTPRF
Gene Symbol
PTPRF
UniProt
Additional LAR Products
Product Documents for Human/Mouse/Rat LAR Antibody
Product Specific Notices for Human/Mouse/Rat LAR Antibody
For research use only
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