Human/Mouse/Rat LMW-PTP/ACP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5075
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 475417
Product Specifications
Immunogen
E. coli-derived recombinant human LMW-PTP/ACP1
Ala2-His158
Accession # P24666
Ala2-His158
Accession # P24666
Specificity
Detects human, mouse, and rat LMW-PTP/ACP1 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat LMW-PTP/ACP1 Antibody
Detection of Human LMW-PTP/ACP1 by Western Blot.
Western blot shows lysates of CHP-100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat LMW-PTP/ACP1 Monoclonal Antibody (Catalog # MAB5075) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for LMW-PTP/ACP1 at approximately 18 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat LMW-PTP/ACP1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: CHP‑100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line
Sample: CHP‑100 human neuroblastoma cell line and HepG2 human hepatocellular carcinoma cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: LMW-PTP/ACP1
Low molecular weight protein tyrosine phosphatase (LMW-PTP), also known as Acid Phosphatase 1 (ACP1), is an 18 kDa cytosolic phosphatase that is unrelated to other tyrosine phosphatases, such as PTP1B. Tyrosine phosphorylation of LMW-PTP increases its activity 20-fold and affects its ability to dephosphorylate targets such as the PDGF receptor. Cancer cells overexpressing LMW-PTP have a higher proliferative rate and generate tumors that are larger than untransfected controls. Colon adenocarcinomas induced by dimethylhydrazine also have elevated levels of LMW-PTP, suggesting that this phosphatase may be a marker for oncogenic transformation. Human LMW-PTP shares 86% and 87% amino acid sequence identity with mouse and rat LMW-PTP, respectively.
Long Name
Low Molecular Weight Phosphotyrosine Protein Phosphotase
Alternate Names
ACP1, HAAP, LMWPTP
Gene Symbol
ACP1
UniProt
Additional LMW-PTP/ACP1 Products
Product Documents for Human/Mouse/Rat LMW-PTP/ACP1 Antibody
Product Specific Notices for Human/Mouse/Rat LMW-PTP/ACP1 Antibody
For research use only
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