Human/Mouse/Rat MKK3/MKK6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2514
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 275922
Product Specifications
Immunogen
E. coli-derived recombinant human MKK3
Met1-Ser347
Accession # P46734
Met1-Ser347
Accession # P46734
Specificity
Detects human MKK3/MKK6 in direct ELISAs and recombinant and endogenous MKK3/MKK6 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat MKK3/MKK6 Antibody
Detection of Human/Mouse/Rat MKK3/MKK6 by Western Blot.
Western blot shows lysates of NRK rat normal kidney cell and C6 rat glioma cell line. PVDF membrane was probed with 1 µg/mL Mouse Anti-Human/Mouse/Rat MKK3/MKK6 Monoclonal Antibody (Catalog # MAB2514) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). For additional reference, recombinant human MKK3 and MKK6 (2 ng/lane) were included. Specific bands for MKK3 and MKK6 was detected at approximately 40 kDa and 38 kDa, respectively (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.Applications for Human/Mouse/Rat MKK3/MKK6 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell and C6 rat glioma cell line
Sample: NRK rat normal kidney cell and C6 rat glioma cell line
Reviewed Applications
Read 1 review rated 4 using MAB2514 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: MKK3/MKK6
Mitogen-activated protein kinase kinase 3 (MKK3), also known as MEK3, and the closely related MKK6 are activated by proinflammatory cytokines and environmental stresses. Activation occurs through phosphorylation by several upstream MAPK kinase kinases, including ASK1 and TAK1. Both MKK3 and MKK6 are dual specificity protein kinases, phosphorylating and activating the p38 MAP kinases at Thr and Tyr positions within the phosphoacceptor sequence Thr-Gly-Tyr.
Long Name
Mitogen-activated Protein Kinase Kinase 3
Alternate Names
Dual specificity mitogen-activated protein kinase kinase 6;MAP2K6;MAPKK6;MEK6;MKK6;PRKMK6;SAPKK3;SAPKK-3
UniProt
Additional MKK3/MKK6 Products
Product Documents for Human/Mouse/Rat MKK3/MKK6 Antibody
Product Specific Notices for Human/Mouse/Rat MKK3/MKK6 Antibody
The purchase of this product conveys to the buyer the limited, non-exclusive, non-transferable right (without the right to resell, repackage, or further sublicense) to use these reagents for research purposes only. No other license is granted to the buyer whether expressly, by implication, by estoppel or otherwise. In particular, the purchase of this product does not include nor carry any right or license to use, develop, or otherwise exploit this product commercially, which includes without limitation, provision of services to a third party, generation of commercial databases, clinical diagnostics or therapeutics, or drug development. This product is sold pursuant to a license from the University of Massachusetts, and the University of Massachusetts reserves all other rights to use these reagents. For information on purchasing a license for uses other than research, please contact the University of Massachusetts, Office of Technology Management, 333 South Street, Suite 400, Shrewsbury, Massachusetts, 01545.
For research use only
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