Human/Mouse/Rat NDFIP1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8246
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human NDFIP1
Ala10-Asp105
Accession # Q9BT67
Ala10-Asp105
Accession # Q9BT67
Specificity
Detects human, mouse and rat NDFIP1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat NDFIP1 Antibody
Detection of Human, Mouse, and Rat NDFIP1 by Western Blot.
Western blot shows lysates of A172 human glioblastoma cell line, SK-Mel-28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1-6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 2 µg/mL of Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for NDFIP1 at approximately 26-28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
NDFIP1 in SH‑SY5Y Human Cell Line.
NDFIP1 was detected in immersion fixed SH-SY5Y human neuroblastoma cell line using Sheep Anti-Human/Mouse/Rat NDFIP1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8246) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.Applications for Human/Mouse/Rat NDFIP1 Antibody
Application
Recommended Usage
Immunocytochemistry
5-15 µg/mL
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Sample: Immersion fixed SH‑SY5Y human neuroblastoma cell line
Western Blot
2 µg/mL
Sample: A172 human glioblastoma cell line, SK‑Mel‑28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1‑6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line
Sample: A172 human glioblastoma cell line, SK‑Mel‑28 human malignant melanoma cell line, bEnd.3 mouse endothelioma cell line, Hepa 1‑6 mouse hepatoma cell line, C6 rat glioma cell line, and NRK rat normal kidney cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: NDFIP1
NDFIP1 (NEDD4 family-interacting protein 1 and NEDD4 WW domain-binding protein 5) is a 26 kDa widely expressed protein. NDFIP1 is thought to be part of a family of integral Golgi membrane proteins. It serves as an adaptor for several E3 ubiquitin ligases including NEDD4 and ITCH, and consequently modulates the stability of their targets. NDFIP1 is genetically linked to human allergic and autoimmune disease, but the cellular mechanism by which these proteins enable foreign and self-antigens to be tolerated is unresolved. Over aa10-105, human NDFIP1 is 95% identical to mouse and rat NDFIP1.
Long Name
Nedd4 Family Interacting Protein 1
Alternate Names
N4WBP5
Gene Symbol
NDFIP1
UniProt
Additional NDFIP1 Products
Product Documents for Human/Mouse/Rat NDFIP1 Antibody
Product Specific Notices for Human/Mouse/Rat NDFIP1 Antibody
For research use only
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