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Human/Mouse/Rat Neurofascin Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3235

R&D Systems, part of Bio-Techne
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AF3235
AF3235-SP

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Mouse, Rat, Transgenic Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Bioassay, Biotinylation, Immunocytochemistry, Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Chicken IgY

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant rat Neurofascin
Ile25-Ala1031
Accession # NP_446361

Specificity

Detects human, mouse, rat Neurofascin in Western blots and rat Neurofascin in direct ELISAs.

Clonality

Polyclonal

Host

Chicken

Isotype

IgY

Scientific Data Images for Human/Mouse/Rat Neurofascin Antibody

Detection of Human, Mouse, and Rat Neurofascin antibody by Western Blot.

Detection of Human, Mouse, and Rat Neurofascin by Western Blot.

Western blot shows lysates of rat brain tissue, mouse brain tissue, and human brain (cerebellum). PVDF Membrane was probed with 0.1 µg/mL of Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) followed by HRP-conjugated Anti-Chicken IgY Secondary Antibody. Specific bands were detected for Neurofascin at approximately 140 and186 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Neurofascin antibody in Rat Brain by Immunohistochemistry (IHC-Fr).

Neurofascin in Rat Brain.

Neurofascin was detected in perfusion fixed frozen sections of rat brain (dorsal root ganglion) using Chicken Anti-Human/Mouse/Rat Neurofascin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3235) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Chicken IgY Secondary Antibody (yellow; Catalog # NL016) and counterstained with DAPI (blue). Specific staining was localized to neuronal cell bodies and Schwann cells (perinodal regions). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat Neurofascin Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of rat brain (dorsal root ganglion)

Western Blot

0.1 µg/mL
Sample: Rat brain tissue, mouse brain tissue, and human brain (cerebellum) tissue

Reviewed Applications

Read 1 review rated 5 using AF3235 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Neurofascin

Neurofascin 155 (NF155) is a type I transmembrane glycoprotein that belongs to the L1CAM family of cell adhesion proteins (1, 2). The rat NF155 cDNA encodes a 1240 amino acid (aa) precursor that contains a 24 aa signal sequence, a 1086 aa extracellular domain (ECD), a 21 aa transmembrane segment, and a 109 aa cytoplasmic domain. The ECD consists of six Ig-like domains and four fibronectin type III repeats, the second of which has an RGD motif. A splice variant of Neurofascin, NF186, lacks the RGD-containing fibronectin type III domain but instead has a mucin-like domain and an additional non-RGD fibronectin type III domain (3). Within shared regions of the ECD, rat NF155 shares 45% and 39% aa sequence identity with rat Nr-CAM and L1CAM, respectively, and 98% aa sequence identity with human and mouse NF155. NF155 is transiently expressed by oligodendrocytes at the onset of axon myelination, whereas NF186 is neuronally expressed in nodes of Ranvier (4‑6). Clustering of NF155 in paranodal oligodengroglia lipid raft domains is stabilized by dimerization of its cytoplasmic domains and association with intracellular ankyrin (6‑9). NF155 interacts with axonal contactin and plays a role in node of Ranvier formation and the establishment of saltatory conduction (5, 9‑12). The ECD of NF155 is cleaved from oligodengroglia membranes by metalloproteases, a process which is required for NF155 transport from the glial cell body to the axoglial junction (13). In addition to distinct expression patterns, Neurofascin isoforms have different functional properties. NF155 promotes neuronal adhesion and neurite outgrowth, whereas NF186 inhibits neuronal adhesion (4, 7, 13).

References

  1. Sherman, D.L. and P.J. Brophy (2005) Nat. Rev. Neurosci. 6:683.
  2. Coman, I. et al. (2005) J. Neurol. Sci. 233:67.
  3. Volkmer, H. et al. (1992) J. Cell Biol. 118:149.
  4. Koticha, D. et al. (2005) Mol. Cell. Neurosci. 30:137.
  5. Collinson, J.M. et al. (1998) Glia 23:11.
  6. Schafer, D.P. et al. (2004) J. Neurosci. 24:3176.
  7. Maier, O. et al. (2005) Mol. Cell. Neurosci. 28:390.
  8. Zhang, X. et al. (1998) J. Biol. Chem. 273:30785.
  9. Sherman, D.L. et al. (2005) Neuron 48:737.
  10. Gollan, L. et al. (2003) J. Cell Biol. 163:1213.
  11. Charles, P. et al. (2002) Curr. Biol. 12:217.
  12. Volkmer, H. et al. (1996) J. Cell Biol. 135:1059.
  13. Maier, O. et al. (2006) Exp. Cell Res. 312:500.

Alternate Names

NFASC, NRCAML

Entrez Gene IDs

23114 (Human); 269116 (Mouse); 116690 (Rat)

Gene Symbol

NFASC

Additional Neurofascin Products

Product Documents for Human/Mouse/Rat Neurofascin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat Neurofascin Antibody

For research use only

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