Human/Mouse/Rat Nrf1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB5306
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Rat
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 492413
Product Specifications
Immunogen
E. coli-derived recombinant human Nrf1
Met1-Lys742
Accession # AAH10623
Met1-Lys742
Accession # AAH10623
Specificity
Detects endogenous human, mouse and rat Nrf1 at 85 kDa in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Nrf1 Antibody
Detection of Human, Mouse, and Rat Nrf1 by Western Blot.
Western blot shows lysates of K562 human chronic myelogenous leukemia cell line, MOLT-4 human acute lymphoblastic leukemia cell line, SH-SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human/Mouse/Rat Nrf1 Monoclonal Antibody (Catalog # MAB5306) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Nrf1 at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Detection of Rat Nrf1 by Western Blot
Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Rat Nrf1 by Western Blot
Effect of myricetin on mitochondrial biogenesis regulators under acute hypoxia detecting by western blotting.(A) Representative images of western blots for genes related to mitochondrial biogenesis in skeletal muscle tissue of rats under acute hypoxia exposure; (B) Representative images of western blots for genes related to mitochondrial biogenesis in L6 muscle cell line. Image collected and cropped by CiteAb from the following publication (https://dx.plos.org/10.1371/journal.pone.0124727), licensed under a CC-BY license. Not internally tested by R&D Systems.Applications for Human/Mouse/Rat Nrf1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3‑Ag rat myeloid cell line
Sample: K562 human chronic myelogenous leukemia cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, SH‑SY5Y human neuroblastoma cell line, A20 mouse B cell lymphoma cell line, and Y3‑Ag rat myeloid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Nrf1
NF-E2-related factor 1 (Nrf1; also transcription factor 11 and LCR-F1) is a ubiquitously expressed transcription factor and member of the bZIP family and the CNC subfamily. Human Nrf1 is 772 amino acids (aa) in length. Like other members of the bZIP family, Nrf1 contains heptad repeats of leucine and hydrophobic residues within a putative amphipathic helical domain of 40 aa and is preceded by a 30 aa domain rich in arginine and lysine residues. A splicing variant produces two isoforms for Nrf1. Isoform 2 has a 30 aa deletion corresponding to aa 242‑271 in isoform 1. Human Nrf1 is 96% and 93% identical to bovine and mouse Nrf1, respectively.
Long Name
NF-E2-related Factor 1
Alternate Names
HBZ17, LCR-F1, NFE2L1, TCF11
Gene Symbol
NFE2L1
UniProt
Additional Nrf1 Products
Product Documents for Human/Mouse/Rat Nrf1 Antibody
Product Specific Notices for Human/Mouse/Rat Nrf1 Antibody
For research use only
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