Human/Mouse/Rat p23/PTGES3 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB10039

R&D Systems, part of Bio-Techne
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MAB10039-100
MAB10039-SP
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 998320

View all p23/PTGES3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant Human p23/PTGES3
Gln21-Glu160
Accession # Q15185

Specificity

Detects human p23/PTGES3 in direct ELISAs. Detects human, mouse, and rat p23/PTGES3 in Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Scientific Data Images for Human/Mouse/Rat p23/PTGES3 Antibody

Detection of Human, Mouse, and Rat p23/PTGES3 antibody by Western Blot.

Detection of Human, Mouse, and Rat p23/PTGES3 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HEK293T human embryonic kidney cell line, NIH-3T3 mouse embryonic fibroblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat p23/PTGES3 Monoclonal Antibody (Catalog # MAB10039) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for p23/PTGES3 at approximately 23 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat p23/PTGES3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, HEK293T human embryonic kidney cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: p23/PTGES3

Prostaglandin E Synthase 3 (also known as Cytosolic prostaglandin E2 synthase, HSP90 co-chaperone, Progesterone receptor complex p23, Telomerase-binding protein p23, or p23) is a glutathione-dependent enzyme found in the cyclooxygenase-1-mediated PGE2 biosynthetic pathway. This protein is highly conserved in eukaryotes and in humans it is expressed in most tissues other than striated muscle. Through its prostaglandin synthase activity, p23 contributes to the production of prostaglandin E2 and has a role in maintenance of tissue homeostasis. In addition to its catalytic activity in the prostaglandin biosynthesis pathway, p23 serves as a co-chaperone to HSP90 (Heat Shock Protein 90) in various biological functions. The p23/HSP90 complex is required for efficient telomerase assembly in vitro and in vivo. It has also been demonstrated that p23 and HSP90 localize to genomic response elements in a hormone-dependent manner, and may promote disassembly of transcriptional regulatory complexes in response to changes in cellular signaling pathways. p23 protein is up-regulated in several cancers, notably breast cancer.

Long Name

Prostaglandin E Synthase 3 (Cytosolic)

Alternate Names

CPGES, PTGES3, TEBP

Entrez Gene IDs

10728 (Human); 56351 (Mouse); 362809 (Rat)

Gene Symbol

PTGES3

UniProt

Q15185

Additional p23/PTGES3 Products

Product Documents for Human/Mouse/Rat p23/PTGES3 Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat p23/PTGES3 Antibody

For research use only

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