Human/Mouse/Rat p27/Kip1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2256
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
CyTOF-ready, Flow Cytometry, Western Blot
Cited:
Pull Down Assay, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human p27
Met1-Thr198
Accession # P46527
Met1-Thr198
Accession # P46527
Specificity
Detects human, mouse and rat p27.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat p27/Kip1 Antibody
Detection of Human p27/Kip1 by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for p27/Kip1 at approximately 30 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of p27/Kip1in Human PBMCs by Flow Cytometry.
Human peripheral blood mononuclear cells were stained with Goat Anti-Human p27/Kip1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2256, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).Applications for Human/Mouse/Rat p27/Kip1 Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Flow Cytometry
2.5 µg/106 cells
Sample: Human peripheral blood mononuclear cells
Sample: Human peripheral blood mononuclear cells
Western Blot
1 µg/mL
Sample: MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line
Sample: MCF-7 human breast cancer cell line and U2OS human osteosarcoma cell line
Reviewed Applications
Read 1 review rated 4 using AF2256 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: p27/Kip1
The cyclin-dependent kinase (cdk) inhibitor p27/Kip1 is a tumor suppressor protein that acts in the nucleus to enforce cell cycle checkpoints. p27/Kip1 binds to and inhibits many cyclin/cdk complexes, including cyclin D/cdk4, cyclin E/cdk2, and cyclin A/cdk2. This inhibition can subsequently block progression through different phases of the cell cycle.
Long Name
p27 Cyclin Dependent Kinase 4 Inhibitor 1B
Alternate Names
CDKN1B, Kip1
Gene Symbol
CDKN1B
UniProt
Additional p27/Kip1 Products
Product Documents for Human/Mouse/Rat p27/Kip1 Antibody
Product Specific Notices for Human/Mouse/Rat p27/Kip1 Antibody
For research use only
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