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Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB7568

R&D Systems, part of Bio-Techne
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MAB7568-100
MAB7568-SP

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 826722

Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse PD-ECGF/Thymidine Phosphorylase
Met1-Pro471
Accession # Q99N42

Specificity

Detects mouse PD-ECGF/Thymidine Phosphorylase in direct ELISAs. Detects human, mouse, and rat PD-ECGF/Thymidine Phosphorylase in Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

Detection of Human, Mouse, and Rat PD-ECGF/Thymidine Phosphorylase antibody by Western Blot.

Detection of Human, Mouse, and Rat PD-ECGF/Thymidine Phosphorylase by Western Blot.

Western blot shows lysates of A431 human epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and H4-II-E-C3 rat hepatoma cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (Catalog # MAB7568) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PD-ECGF/Thymidine Phosphorylase at approximately 49 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PD-ECGF/Thymidine Phosphorylase antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

PD-ECGF/Thymidine Phosphorylase in Mouse Embryo.

PD-ECGF/Thymidine Phosphorylase was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Monoclonal Antibody (Catalog # MAB7568) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Applications for Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

Application
Recommended Usage

Immunohistochemistry

8-25 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (15 d.p.c.)

Western Blot

2 µg/mL
Sample: A431 human epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and H4‑II‑E‑C3 rat hepatoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PD-ECGF/Thymidine Phosphorylase

TYMP (Thymidine phosphorylase/TP; also [PD]-ECGF/platelet-derived endothelial cell growth factor, gliostatin and TdRPase) is a 50-55 kDa member of the pyrimidine-nucleoside phosphorylase family of enzymes. TYMP/TP is both a cytosolic and secreted molecule that is expressed by a variety of cell types, including, macrophages, hepatocytes, endometrial gland epithelium, vascular smooth muscle and endothelial cells. It has also been found in select tumor cell types, and via an angiogenic activity, has been proposed to promote tumor growth. TYMP converts thymidine to thymine and 2-deoxy-ribose-1P, and it is the 2-deoxy-ribose component that is believed to promote endothelial cell migration (but not proliferation). This may be due to the fact that 2-deoxy-ribose induces reactive oxygen species, which drive the production of angiogenic factors, and that 2-deoxy-ribose also activates endothelial cell integrins. Mouse TYMP is 471 amino acids (aa) in length. It contains a phosphorylase domain (aa 96-348), followed by a C-terminal region (aa 374-448). Although TYMP circulates, there is no definitive signal sequence. TYMP is known to form homodimers. Full-length mouse TYMP (aa 1-471) shares 80% and 92% aa sequence identity with human and rat TYMP, respectively.

Long Name

Platelet-derived Endothelial Cell Growth Factor

Alternate Names

dThdPase, ECGF1, Gliostatin, MEDPS1, MNGIE, MTDPS1, PDECGF, TdRPase, Thymidine Phosphorylase, TPase, TYMP

Entrez Gene IDs

1890 (Human); 72962 (Mouse)

Gene Symbol

TYMP

UniProt

Additional PD-ECGF/Thymidine Phosphorylase Products

Product Documents for Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human/Mouse/Rat PD-ECGF/Thymidine Phosphorylase Antibody

For research use only

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