Human/Mouse/Rat PDCD5 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7325
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 728906
Product Specifications
Immunogen
E. coli-derived recombinant human PDCD5
Ala2-Tyr125
Accession # O14737
Ala2-Tyr125
Accession # O14737
Specificity
Detects human PDCD5 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant
human PDCD6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat PDCD5 Antibody
Detection of Human, Mouse, and Rat PDCD5 by Western Blot.
Western blot shows lysates of HEK293 human embryonic kidney cell line, EL-4 mouse lymphoblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD5 Polyclonal Antibody (Catalog # MAB7325) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD5 at approximately 15 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
PDCD5 in Human Stomach.
PDCD5 was detected in immersion fixed paraffin-embedded sections of human stomach using Mouse Anti-Human/Mouse/Rat PDCD5 Monoclonal Antibody (Catalog # MAB7325) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of epithelial cells in gastric glands. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.Applications for Human/Mouse/Rat PDCD5 Antibody
Application
Recommended Usage
Immunohistochemistry
8-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach
Sample: Immersion fixed paraffin-embedded sections of human stomach
Western Blot
0.5 µg/mL
Sample: HEK293 human embryonic kidney cell line, EL‑4 mouse lymphoblast cell line, and L6 rat myoblast cell line
Sample: HEK293 human embryonic kidney cell line, EL‑4 mouse lymphoblast cell line, and L6 rat myoblast cell line
Reviewed Applications
Read 1 review rated 5 using MAB7325 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDCD5
Long Name
Programmed Cell Death 5
Alternate Names
PD5, TFAR19
Gene Symbol
PDCD5
UniProt
Additional PDCD5 Products
Product Documents for Human/Mouse/Rat PDCD5 Antibody
Product Specific Notices for Human/Mouse/Rat PDCD5 Antibody
For research use only
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