Human/Mouse/Rat PDCD6 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB6015
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 722232
Product Specifications
Immunogen
E. coli-derived recombinant human PDCD6
Met1-Val189
Accession # O75340
Met1-Val189
Accession # O75340
Specificity
Detects human PDCD6 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant
human (rh) PDCD4 or rhPDCD5 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat PDCD6 Antibody
Detection of Human, Mouse, and Rat PDCD6 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, Hepa 1-6 mouse hepatoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human/Mouse/Rat PDCD6 Monoclonal Antibody (Catalog # MAB6015) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PDCD6 at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PDCD6 Antibody
Application
Recommended Usage
Western Blot
0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and NRK rat normal kidney cell line
Sample: HeLa human cervical epithelial carcinoma cell line, Hepa 1‑6 mouse hepatoma cell line, and NRK rat normal kidney cell line
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PDCD6
Long Name
Programmed Cell Death 6
Alternate Names
ALG2, PEF1B
Entrez Gene IDs
10016 (Human)
Gene Symbol
PDCD6
UniProt
Additional PDCD6 Products
Product Documents for Human/Mouse/Rat PDCD6 Antibody
Product Specific Notices for Human/Mouse/Rat PDCD6 Antibody
For research use only
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