Human/Mouse/Rat PDK-1 Antibody

Detection of Human, Mouse, and Rat PDK‑1 by Western Blot.

Western blot shows lysates of Jurkat human acute T cell leukemia cell line, HepG2 human hepatocellular carcinoma cell line, Daudi human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and C6 rat glioma cell line. PVDF Membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PDK-1 Monoclonal Antibody (Catalog # MAB864) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). Specific bands were detected for PDK-1 at approximately 58-68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

PDK‑1 in HeLa Human Cell Line.

PDK-1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Mouse Anti-Human/Mouse/Rat PDK-1 Monoclonal Antibody (Catalog # MAB864) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human PDK-1 by Simple Western^TM.

Simple Western shows lysates of Daudi human Burkitt's lymphoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.5 mg/ml. A specific band was detected for PDK-1 at approximately 60 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat PDK-1 Monoclonal Antibody (Catalog # MAB864). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.