Human/Mouse/Rat Peroxiredoxin 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3489
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Immunocytochemistry, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 477719
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119
Met1-Asn198
Accession # P32119
Specificity
Detects human, mouse, and rat Peroxiredoxin 2 in Western blots. In Western blots, approximately 25%-40% cross-reactivity with recombinant human (rh) Peroxiredoxin 1 and 4 is observed, and less than 10% cross-reactivity with rhPeroxiredoxin 3, 5, and 6 is observed. In direct ELISAs, approximately 15%-25% cross-reactivity with rhPeroxiredoxin 1 and 4 is observed, and less than 5% cross-reactivity with rhPeroxiredoxin 3, 5, or 6 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat Peroxiredoxin 2 Monoclonal Antibody (Catalog # MAB3489) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Sample: NRK rat normal kidney cell, Raji human Burkitt's lymphoma cell line, and NIH-3T3 mouse embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 3 reviews rated 3.7 using MAB3489 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 2
Alternate Names
NKEF-B, PRDX2, PRX2, TDPX1, TSA
Gene Symbol
PRDX2
UniProt
Additional Peroxiredoxin 2 Products
Product Documents for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 2 Antibody
For research use only
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