Human/Mouse/Rat Peroxiredoxin 2 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF3489
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human, Mouse
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Peroxiredoxin 2
Met1-Asn198
Accession # P32119
Met1-Asn198
Accession # P32119
Specificity
The antibody detects endogenous human, mouse and rat Peroxiredoxin 2 in in Western blots. In Western blots, approximately25% cross‑reactivity with recombinant human (rh) Peroxiredoxin 1 and rhPeroxiredoxin 4 is observed, approximately 10% cross-reactivity with rhPeroxiredoxin 3 is observed, and less than 1% cross‑reactivity with rhPeroxiredoxin 5 and rhPeroxiredoxin 6 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Detection of Human/Mouse/Rat Peroxiredoxin 2 by Western Blot.
Western blot shows lysates of Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Peroxiredoxin 2 at approximately 24 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 2.
Detection of Human Peroxiredoxin 2 by Simple WesternTM.
Simple Western shows lysates of Exosome Standard (PC-3) (NBP2-49856) and Raji human Burkitt's lymphoma cell line, loaded at 0.5 mg/ml. A specific band was detected for Peroxiredoxin 2 at approximately 26 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Mouse/Rat Peroxiredoxin 2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3489). This experiment was conducted under reducing conditions and using the 2-40kDa separation system.Applications for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Exosome Standard (PC-3) (Catalog # NBP2-49856) and Raji human Burkitt's lymphoma cell line
Sample: Exosome Standard (PC-3) (Catalog # NBP2-49856) and Raji human Burkitt's lymphoma cell line
Western Blot
0.2 µg/mL
Sample: Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line
Sample: Raji human Burkitt's lymphoma cell line, A20 mouse B cell lymphoma cell line, and NRK rat normal kidney cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Peroxiredoxin 2
Alternate Names
NKEF-B, PRDX2, PRX2, TDPX1, TSA
Gene Symbol
PRDX2
UniProt
Additional Peroxiredoxin 2 Products
Product Documents for Human/Mouse/Rat Peroxiredoxin 2 Antibody
Product Specific Notices for Human/Mouse/Rat Peroxiredoxin 2 Antibody
For research use only
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