Human/Mouse/Rat PGK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5455
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human PGK1
Met1-Ile417
Accession # P00558
Met1-Ile417
Accession # P00558
Specificity
Detects endogenous human, mouse and rat PGK1 in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PGK1 Antibody
Detection of Human/Mouse/Rat PGK1 by Western Blot.
Western blot shows lysates of A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PGK1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5455) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PGK1 at approximately 43 to 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PGK1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line
Sample: A431 human epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, U937 human histiocytic lymphoma cell line, BaF3 mouse pro-B cell line, A20 mouse B cell lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and Y3-Ag rat myeloid cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PGK1
Phosphoglycerate kinase-1 (PGK1) is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3 phosphoglycerate. The gene encoding PGK1 is X-linked. Mutations of this gene may cause phosphoglycerate kinase deficiency, which is characterized by hemolytic anemia, muscle stiffness and mental retardation (1‑3). PGK1 is induced by oxidative stress through the induction of hypoxia-inducible factor 1 alpha and is a potential biomarker and therapeutic target for cancer (4‑7).
References
- Beutler, E. (2002) Br. J. Haematol. 136:3.
- Flanagan, J.M. et al. (2006) Br. J. Haematol. 134:233.
- Svaasand, E.K. et al. (2007) Muscle Nerve. 36:579.
- Hwang, T.L. et al. (2006) Proteomics. 6:2259.
- Wang, J. et al. (2007) Cancer Res. 67:149.
- Zieker, D. et al. (2008) Cell Physiol. Biochem. 21:429.
- Jang, C.H. et al. (2008) Biosci. Biotechnol. Biochem. 72:1799.
Long Name
Phosphoglycerate Kinase-1
Alternate Names
MIG10, PGKA, PRP2
Gene Symbol
PGK1
UniProt
Additional PGK1 Products
Product Documents for Human/Mouse/Rat PGK1 Antibody
Product Specific Notices for Human/Mouse/Rat PGK1 Antibody
For research use only
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