Human/Mouse/Rat Phospho-Chk1 (S317) Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2054
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Simple Western, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Phosphopeptide containing human Chk1 S317 site
Specificity
Detects human, mouse, and rat Chk1 when phosphorylated at S317 in Western blots. Does not recognize Chk1 when unphosphorylated at S317.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody
Detection of Human Phospho-Chk1 (S317) by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or exposed (+) to 50 J/m2UV-C for the indicated time. PVDF membrane was probed with 1 µg/mL Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band for Phospho-Chk1 (S317) was detected at approximately 56 kDa (as indicated). The phospho-specificity of this antibody was supported by decreased labeling following treatment with 600 U lambda-phosphatase (lambda-PPase) for 1 hour. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Phospho-Chk1 (S317) by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 50 J/m2ultraviolet light (UV) for 2 hours, loaded at 0.2 mg/mL. A specific band was detected for Phospho-Chk1 (S317) at approximately 60 kDa (as indicated) using 10 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-Chk1 (S317) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2054). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with ultraviolet light (UV)
Sample: HeLa human cervical epithelial carcinoma cell line treated with ultraviolet light (UV)
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line exposed to UV-C
Sample: HeLa human cervical epithelial carcinoma cell line exposed to UV-C
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Chk1
The Chk1 checkpoint kinase is an integral member of a signaling cascade that controls cell cycle progression. In response to genotoxic or replicative stress, Chk1 is phosphorylated by ATM or ATM-related kinases (ATR) at S317. In turn, Chk1 phosphorylates downstream effectors, such as p53 or the Cdc25 phosphatases to halt cell cycle progression and allow time for repair of incurred damage.
Long Name
Checkpoint Kinase 1
Alternate Names
CHEK1
Gene Symbol
CHEK1
Additional Chk1 Products
Product Documents for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody
Product Specific Notices for Human/Mouse/Rat Phospho-Chk1 (S317) Antibody
For research use only
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