Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB79671
Recombinant Monoclonal Antibody.
Key Product Details
Validated by
Biological Validation
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1024A
Product Specifications
Immunogen
Phosphopeptide containing the human RSK1 S380 site
Accession # Q15418
Accession # Q15418
Specificity
Detects human, mouse, and rat RSK1 when phosphorylated at S380 in Western blots. Expected to detect RSK2, RSK3, and RSK4 phosphorylated at S386, S377, and S389, respectively, based on sequence homology.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
Detection of Human Phospho-RSK1 (S380) by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA or PMA and Ionomycin for 20 minutes. PVDF membrane was probed with 0.1 µg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-RSK1 (S380) at approximately 93 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
RSK1 in HeLa Human Cell Line.
RSK1 was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line, unstimulated (panels B and D) or stimulated with PMA (panels A and C), using Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671) at 25 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red, upper panels; Catalog # NL004) and counterstained with DAPI (blue, lower panels). Specific staining was localized to plasma membrances and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Phospho-RSK1 (S380) by Simple WesternTM.
Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes, and loaded at 0.2 mg/mL. A specific band was detected for Phospho-RSK1 (S380) at approximately 90 kDa (as indicated) using 1 μg/mL of Rabbit Anti-Human/Mouse/Rat Phospho-RSK1 (S380) Monoclonal Antibody (Catalog # MAB79671). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
Application
Recommended Usage
Immunocytochemistry
5-25 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with PMA
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line stimulated with PMA
Simple Western
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Sample: HeLa human cervical epithelial carcinoma cell line treated with PMA
Western Blot
0.1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line treated with PMA or PMA and Ionomycin
Sample: Jurkat human acute T cell leukemia cell line and HeLa human cervical epithelial carcinoma cell line treated with PMA or PMA and Ionomycin
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB79671 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK1
RSK1 (ribosomal S6 kinase 1), gene name RPS6KA1 (ribosomal protein S6 kinase alpha1), also called p90RSK1 (90 kDa RSK1) or MAPKAPK 1a (MAPK-activated protein kinase 1a), is a widely expressed member of the RSK family of growth factor-regulated serine/threonine kinases. RSK proteins contain two non-identical kinase catalytic domains, and mediate activation of mitogen-activated kinase (MAPK) cascades and stimulation of cell proliferation and differentiation. Autophosphorylation on S380 allows binding of PDPK1, leading to further phosphorylation and full activation of RSK1 kinase activity. Human, mouse and rat RSK1 share 98% aa sequence identity, and are identical within the region of the peptide immunogen.
Long Name
Ribosomal Protein S6 Kinase I
Alternate Names
HU-1, MAPKAPL1A, RPS6KA1
Gene Symbol
RPS6KA1
UniProt
Additional RSK1 Products
Product Documents for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
Product Specific Notices for Human/Mouse/Rat Phospho-RSK1 (S380) Antibody
For research use only
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