Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF2998
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PI 3-Kinase p85 alpha
Leu328-Tyr431
Accession # P27986
Leu328-Tyr431
Accession # P27986
Specificity
Detects endogenous human, mouse and rat PI 3-Kinase p85 alpha in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Detection of Human/Mouse/Rat PI 3-Kinase p85 alpha by Western Blot.
Western blot shows lysates of Jurkat human acute T cell leukemia cell line, mouse brain tissue and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PI 3‑Kinase p85 alpha Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2998) followed by HRP‑conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PI 3‑Kinase p85 alpha at approximately 85 kDa (as indicated). This experiment was conducted using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Jurkat human acute T cell leukemia cell line, mouse brain tissue, and rat brain tissue
Sample: Jurkat human acute T cell leukemia cell line, mouse brain tissue, and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PI 3-Kinase p85 alpha
Class I phosphoinositide 3-kinases (PI 3-Kinases) are heterodimeric proteins that consist of a catalytic subunit of 110‑120 kDa and an associated regulatory subunit. p85 alpha is one of the regulatory subunits that associate with the class IA PI 3-Kinases. Composed of one SH3 and two SH2 domains, p85 alpha functions as an adapter, coupling catalytic p110 to activated receptor tyrosine kinases.
Long Name
PI 3-Kinase p85 alpha
Alternate Names
GRB1, PI 3Kinase p85 alpha, PIK3R1
Entrez Gene IDs
5295 (Human)
Gene Symbol
PIK3R1
UniProt
Additional PI 3-Kinase p85 alpha Products
Product Documents for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
Product Specific Notices for Human/Mouse/Rat PI 3-Kinase p85 alpha Antibody
For research use only
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