Human/Mouse/Rat PKA C alpha Antibody
R&D Systems, part of Bio-Techne | Catalog # AF4268
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKA C alpha
Met1-Phe351
Accession # P17612
Met1-Phe351
Accession # P17612
Specificity
Detects human, mouse, and rat PKA C alpha in Western blots. In Western blots, less than 1% cross-reactivity with recombinant human (rh) PKA C beta or rhPKA C delta is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKA C alpha Antibody
Detection of Human/Mouse/Rat PKA C alpha by Western Blot.
Western blot shows lysates of MCF-7 human breast cancer cell line. PVDF membrane was probed with 2 µg/mL Goat Anti-Human/Mouse/Rat PKA Ca Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4268) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, Recombinant Human Active PKA C alpha (Catalog # 4268-KS), Recombinant Human Active PKA C beta (Catalog # 4596-KS), and recombinant human PKA C gamma (3 ng/lane) were included. A specific band for PKA C alpha was detected at approximately 42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.Applications for Human/Mouse/Rat PKA C alpha Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: MCF-7 human breast cancer cell line
Sample: MCF-7 human breast cancer cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using AF4268 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKA C alpha
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit alpha
Alternate Names
Cs, Pkaca, PKCA1, PKCD, PRKACA
Gene Symbol
PRKACA
UniProt
Additional PKA C alpha Products
Product Documents for Human/Mouse/Rat PKA C alpha Antibody
Product Specific Notices for Human/Mouse/Rat PKA C alpha Antibody
For research use only
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