Recombinant Human Active PKA C beta Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 4596-KS
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 9 (baculovirus)-derived human PKA C beta protein
Purity
>80%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain at 5 μg per lane.
N-terminal Sequence Analysis
Using an N-terminal GST tag
SDS-PAGE
65 kDa
Activity
The specific activity of PKA C beta is typically 423-573 nmol/min/mg using a CREBtide synthetic peptide substrate.
Scientific Data Images for Recombinant Human Active PKA C beta Protein, CF
Recombinant Human Active PKA C beta Protein SDS-PAGE.
The approximate molecular weight is 65 kDa and the purity is > 80%.Formulation, Preparation and Storage
4596-KS
| Formulation | Supplied in 50 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.25 mM DTT, 0.1 mM EDTA, 10 mM Glutathione, 0.1 mM PMSF, and 25% Glycerol. |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | This product is stable at ≤ ‑70 °C for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles. |
Background: PKA C beta
The catalytic subunit C-beta of PKA (PKA C beta) is a member of the Ser/Thr protein kinase family (the PKA catalytic subunit consists of three gene products: C alpha, C beta, and C gamma) and has been assigned to human chromosome region 1p36.1 (1). PKA C beta is derived from a gene distinct from C alpha and shows tissue-specific expression. At the amino acid level C alpha and C beta showed 93% homology.
References
- Simard, J. et al. (1992) Human Genetics 88:653.
Long Name
cAMP-dependent Protein Kinase Catalytic Subunit beta
Alternate Names
cAPKbeta, PRKACB
Gene Symbol
PRKACB
UniProt
Additional PKA C beta Products
Product Documents for Recombinant Human Active PKA C beta Protein, CF
Product Specific Notices for Recombinant Human Active PKA C beta Protein, CF
For research use only
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