Human/Mouse/Rat PKC gamma Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5130
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human PKC gamma
Arg621-Met697
Accession # P05129
Arg621-Met697
Accession # P05129
Specificity
Detects endogenous human, mouse and rat PKC gamma in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat PKC gamma Antibody
Detection of Human/Mouse/Rat PKC gamma by Western Blot.
Western blot shows lysates of human, mouse, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PKC gamma Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5130) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for PKC gamma at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PKC gamma Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Mouse, human, and rat brain tissue
Sample: Mouse, human, and rat brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PKC gamma
PKC gamma (protein kinase C-gamma) is a 78-80 kDa member of the PKC subfamily, AGC Ser/Thr protein kinase family of enzymes. It is a Ca++-activated and phospholipid-dependent neuronal enzyme that catalyzes the phosphorylation of multiple proteins. Human PKC gamma is 697 amino acids (aa) in length. It contains two general regions: a lipid and Ca++-binding regulatory region (aa 35‑150 and 170‑260, respectively), and an ATP-binding catalytic region (aa 351‑614). PDK1-mediated phosphorylation of PKC gamma on Thr514 activates the enzyme. In addition, oxidative stress also mediates activation by inducing interchain disulfide-bond homodimerization. Over aa 621‑697, human PKC gamma is 99% and 95% aa identical to mouse and canine PKC gamma, respectively. PKC gamma is predominantly expressed in brain tissue.
Long Name
Protein Kinase C gamma
Alternate Names
PKCC, PKCG, PRKCG, SCA14
Gene Symbol
PRKCG
UniProt
Additional PKC gamma Products
Product Documents for Human/Mouse/Rat PKC gamma Antibody
Product Specific Notices for Human/Mouse/Rat PKC gamma Antibody
For research use only
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