Human/Mouse/Rat PLC-gamma 1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB32881
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # 479819
Product Summary for Human/Mouse/Rat PLC-gamma 1 Antibody
Immunogen
E. coli-derived recombinant human PLC-gamma 1
Phe550-His663
Accession # P19174
Phe550-His663
Accession # P19174
Specificity
Detects endogenous human, mouse, and rat PLC-gamma 1.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Scientific Data Images for Human/Mouse/Rat PLC-gamma 1 Antibody
Detection of Human/Mouse/Rat PLC-gamma 1 by Western Blot.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat PLC- gamma1 Monoclonal Antibody (Catalog # MAB32881) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for PLC- gamma1 at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat PLC-gamma 1 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and mouse brain tissue
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB32881 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: PLC-gamma 1
PLC-gamma 1 (phospholipase C-gamma 1) cleaves the polar head group from the membrane lipid phosphatidyl-inositol 4,5-biphosphate. The consequence of this reaction is the generation of two secondary messengers, inositol 1,4,5-trisphosphate and diacylglycerol, which mediate the mobilization of intracellular calcium and the activation of protein kinase C, respectively. Members of the PLC family are regulated by different signaling pathways. PLC-gamma 1 is regulated through receptor and non-receptor tyrosine kinases, while the PLC-beta isoforms are regulated by G-protein coupled receptors.
Long Name
Phospholipase C gamma 1
Alternate Names
PLCG1, PLCgamma 1, PPLCA
Gene Symbol
PLCG1
UniProt
Additional PLC-gamma 1 Products
Product Documents for Human/Mouse/Rat PLC-gamma 1 Antibody
Product Specific Notices for Human/Mouse/Rat PLC-gamma 1 Antibody
For research use only
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