Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6340
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived human POR/Cytochrome P450 Reductase
Arg45-Ser677
Accession # P16435
Arg45-Ser677
Accession # P16435
Specificity
Detects recombinant human POR/Cytochrome P450 Reductase in direct ELISAs and human, mouse, and rat POR/Cytochrome P450 Reductase in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody
Detection of Human, Mouse, and Rat POR/ Cytochrome P450 Reductase by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human/Mouse/Rat POR/Cytochrome P450 Reductase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6340) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for POR/Cytochrome P450 Reductase at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody
Application
Recommended Usage
Western Blot
0.5 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue
Sample: HepG2 human hepatocellular carcinoma cell line, mouse kidney tissue, and rat kidney tissue
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: POR/Cytochrome P450 Reductase
NADPH-Cytochrome P450 Reductase (P450R) is an essential component of the cytochrome P450 monooxygenase system of eukaryotic cells (1). P450R is anchored in the endoplasmic reticulum membrane with its catalytic domain residing in the cytosol. P450R is a flavoprotein, containing one molecule each of FMN and FAD, which are essential for the transfer of electrons from NADPH to the cytochromes P450 (2). This reduction is necessary for cytochromes P450 to perform each cycle of oxidation. P450R is also capable of transferring electrons to cytochrome b5, heme oxygenase, the fatty acid elongation system, and other proteins. Mutations of P450R can result in disordered steroidogenesis and Antley-Bixler syndrome.
References
- Philips, A.H. and R.G. Langdon (1962) J. Biol. Chem. 237:2652.
- Iyanagi, T. and H.S. Mason (1973) Biochemistry 12:2291.
- Flueck C.E. et al. (2004) Nat. Genet. 36:228.
Long Name
NADPH Cytochrome P450 Reductase
Alternate Names
CPR, CYPOR, Cytochrome P450 Reductase, P450R
Entrez Gene IDs
5447 (Human)
Gene Symbol
POR
UniProt
Additional POR/Cytochrome P450 Reductase Products
Product Documents for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody
Product Specific Notices for Human/Mouse/Rat POR/Cytochrome P450 Reductase Antibody
For research use only
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