Human/Mouse/Rat PTP mu/PTPRM Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4446

R&D Systems, part of Bio-Techne
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MAB4446
MAB4446-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 436502

View all PTP mu/PTPRM Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human PTPμ/PTPRM
Glu21-Lys742
Accession # P28827

Specificity

Detects human, mouse, and rat PTPμ/PTPRM in Western blots. In Western blots, no cross-reactivity with recombinant human PTPRK, PTPRT, PTPRD, PTPRG, PTPRF (LAR), or DEP1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human/Mouse/Rat PTP mu/PTPRM Antibody

Detection of Human PTPµ/PTPRM antibody by Western Blot.

Detection of Human PTPμ/PTPRM by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, and CHP-100 human neuroblastoma cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat PTPµ/PTPRM Monoclonal Antibody (Catalog # MAB4446) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for PTPµ/PTPRM at approximately 280 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat PTP mu/PTPRM Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, and CHP-100 human neuroblastoma cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTP mu/PTPRM

Protein Tyrosine Phosphatase, receptor type M (PTPRM), also called PTPμ, PTPRμ, and RPTPμ, is expressed at highest levels in pulmonary vascular epithelia, where interactions with cadhedrins are believed to be important in regulating barrier permeability. The MAM and Fibronectin III (FNIII) domains on the extracellular side of PTPRM bind between cells, affecting adhesion and contact inhibition. Culturing cells to high density concentrates PTPRM at sites of tight contact and induces proteolytic cleavage of the 100 kDa extracellular domain.

Long Name

Protein Tyrosine Phosphatase Receptor-type M

Alternate Names

PTPRL1, PTPRM, R-PTP-mu

Entrez Gene IDs

5797 (Human); 19274 (Mouse); 29616 (Rat)

Gene Symbol

PTPRM

UniProt

P28827

Additional PTP mu/PTPRM Products

Product Documents for Human/Mouse/Rat PTP mu/PTPRM Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat PTP mu/PTPRM Antibody

For research use only

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