Human/Mouse/Rat Ras-GAP Antibody
R&D Systems, part of Bio-Techne | Catalog # AF5094
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Product Specifications
Immunogen
E. coli-derived recombinant human Ras-GAP
Trp181-Val272
Accession # P20936
Trp181-Val272
Accession # P20936
Specificity
Detects endogenous human, mouse, and rat Ras-GAP in Western blots.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for Human/Mouse/Rat Ras-GAP Antibody
Detection of Human/Mouse/Rat Ras-GAP by Western Blot.
Western blot shows lysates of TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL of Human/Mouse/Rat Ras-GAP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5094) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for Ras-GAP at approximately 120 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat Ras-GAP Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Sample: TT human medullary thyroid cancer cell line, C2C12 mouse myoblast cell line, and Rat-2 rat embryonic fibroblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ras-GAP
Ras-GAP (Ras GTPase-activating protein) interacts with Ras to stimulate the otherwise weak intrinsic GTPase activity of Ras and to promote the return of Ras to an inactive GDP-bound state. Thus, Ras-GAP is a negative regulator of Ras. Human Ras-GAP is 1047 amino acids in length. The N-terminal region contains two SH2 domains and an intervening SH3 domain, while the C-terminal half of Ras-GAP is sufficient to accelerate Ras GTPase activity.
Long Name
GTPase activating protein
Alternate Names
CM-AVM, PKWS, RASA1, RasGAP
Gene Symbol
RASA1
UniProt
Additional Ras-GAP Products
Product Documents for Human/Mouse/Rat Ras-GAP Antibody
Product Specific Notices for Human/Mouse/Rat Ras-GAP Antibody
For research use only
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