Human/Mouse/Rat ROCK1 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB4590
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 701118
Product Specifications
Immunogen
E. coli-derived recombinant human ROCK1
Ala629-Thr829
Accession # Q13464
Ala629-Thr829
Accession # Q13464
Specificity
Detects human ROCK1 in direct ELISAs and Western blots.
In Western blots, no cross-reactivity
with recombinant human ROCK2 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human/Mouse/Rat ROCK1 Antibody
Detection of Human, Mouse, and Rat ROCK1 by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC-12 rat adrenal pheochromocytoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ROCK1 Monoclonal Antibody (Catalog # MAB4590) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ROCK1 at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human/Mouse/Rat ROCK1 Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line, C2C12 mouse myoblast cell line, and PC‑12 rat adrenal pheochromocytoma cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 1 review rated 5 using MAB4590 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ROCK1
References
- Zhao, Y.M. et al. (2004) Dev. Biol. 275:183.
- Zhang, C. et al. (2006) FASEB J. 20:916.
Long Name
Rho-associated, Coiled-Coil Containing Protein Kinase 1
Alternate Names
p160ROCK, ROK beta
Gene Symbol
ROCK1
UniProt
Additional ROCK1 Products
Product Documents for Human/Mouse/Rat ROCK1 Antibody
Product Specific Notices for Human/Mouse/Rat ROCK1 Antibody
For research use only
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