Human/Mouse/Rat RSK Pan Specific Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB2056
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Simple Western, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # 792921
Product Specifications
Immunogen
synthetic peptide corresponding to Lys171-Asp193 of human RSK2
Accession # P51821
Accession # P51821
Specificity
Detects human, mouse, and rat RSK1, 2, 3, and 4 in Western blots.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Human/Mouse/Rat RSK Pan Specific Antibody
Detection of Human, Mouse, and Rat RSK by Western Blot.
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for RSK at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human RSK by Simple WesternTM.
Simple Western lane view shows lysates of HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for RSK at approximately 86 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human/Mouse/Rat RSK Pan Specific Monoclonal Antibody (Catalog # MAB2056). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human/Mouse/Rat RSK Pan Specific Antibody
Application
Recommended Usage
Simple Western
20 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Sample: HepG2 human hepatocellular carcinoma cell line and HeLa human cervical epithelial carcinoma cell line
Western Blot
1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Sample: HepG2 human hepatocellular carcinoma cell line, NIH‑3T3 mouse embryonic fibroblast cell line, and L6 rat myoblast cell line
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: RSK
Long Name
Ribosomal Protein S6 Kinase II
Alternate Names
HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1
UniProt
Additional RSK Products
Product Documents for Human/Mouse/Rat RSK Pan Specific Antibody
Product Specific Notices for Human/Mouse/Rat RSK Pan Specific Antibody
For research use only
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