Human/Mouse/Rat RSK Pan Specific Antibody

R&D Systems, part of Bio-Techne | Catalog # AF2056

R&D Systems, part of Bio-Techne
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AF2056
AF2056-SP
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

View all RSK Antibodies »

Product Specifications

Immunogen

Human RSK2 synthetic peptide

Specificity

Detects endogenous human, mouse and rat RSK family isoforms in Western blots.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Human/Mouse/Rat RSK Pan Specific Antibody

Detection of Human and Mouse RSK antibody by Western Blot.

Detection of Human and Mouse RSK by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat RSK Pan Specific, Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2056) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for RSK Pan Specific, at approximately 90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human/Mouse/Rat RSK Pan Specific Antibody

Application
Recommended Usage

Western Blot

0.5 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, HepG2 human hepatocellular carcinoma cell line, KG-1 human acute myelogenous leukemia cell line, TS1 mouse helper T cell line, and C2C12 mouse myoblast cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RSK

This peptide sequence is identical to amino acids 162‑184 of human RSK3 (RPS6KA2). The sequence differs at one position from amino acids 165‑187 of human RSK1 (RPS6KA1) and at two positions from amino acids 176‑198 of human RSK4 (RPS6KA6). The immunogen is also highly homologous to the corresponding sequences of mouse and rat RSK family isoforms.

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

HU-1;MAPKAPK1;MAPKAPK1A;p90Rsk;Ribosomal protein S6 kinase alpha-1;RPS6KA1;RSK;RSK1

Additional RSK Products

Product Documents for Human/Mouse/Rat RSK Pan Specific Antibody

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human/Mouse/Rat RSK Pan Specific Antibody

For research use only

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